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. 2019 Jul 23;9:10607. doi: 10.1038/s41598-019-46840-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TLC1 levels are decreased in Ku mutants. (A) Quantification of TLC1 RNA in asynchronous wild type (WT), yku70-R456E, yku70Δ, and yku80-135i strains by RT-qPCR in four independent experiments. TLC1 levels were quantified relative to actin RNA and normalized to WT. Error bars represent ±1 standard deviation (SD). p values adjusted for multiple comparisons are shown. (B) Analysis of TLC1 levels by northern blotting of asynchronous WT, yku70-R456E, yku70Δ, yku80-135i, yku80Δ and tlc1Δ strains. Blots were also probed with ACT1 for loading. Full-length blots are presented in Supplementary Fig. S7. (C) Quantification of three independent northern blotting experiments. Error bars represent ±1 SD. p values adjusted for multiple comparisons.