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. 2019 Jul 23;9:10607. doi: 10.1038/s41598-019-46840-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TLC1 levels are not stabilized in Ku mutant strains expressing CDC13-EST1 fusions. (A) Analysis of TLC1 levels by northern blotting of 1X-4X serial streakouts of cdc13Δ (WT), cdc13Δ yku70-R456E (yku70-R456E), and cdc13Δ yku70Δ (yku70Δ) strains expressing either Cdc13 or a Cdc13-Est1 fusion. Blots were also probed with ACT1 for loading. Full-length blots are presented in Supplementary Fig. S11. (B) Quantification of the mean from three independent northern blotting experiments. Error bars represent ± standard error of the mean (SEM).