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. 2019 Jul 23;9:10607. doi: 10.1038/s41598-019-46840-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Soluble Est1:Cdc13 complexes increase in the absence of Ku. (A) Co-immunoprecipitation of Est1-myc with Cdc13-FLAG. Anti-FLAG immunoprecipitations were performed with asynchronous whole cell lysates of indicated strains. Immunoprecipitates and inputs were analyzed by western blotting with α-myc (Est1) and α-FLAG (Cdc13). Inputs were also probed with α-PGK for loading. Full-length blots are presented in Supplementary Figs S17 and S18. (B) Quantification of mean Est1-myc association relative to Cdc13-FLAG pull-down in five independent experiments for each set of genotypes. Error bars represent ± SD and p values adjusted for multiple comparisons are shown.