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. 2019 Jul 23;9:10646. doi: 10.1038/s41598-019-47123-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Directed differentiation protocol improves RPE differentiation. (A) Schematic representation of the directed differentiation protocol (black star: cell contaminants). (B) Representative macroscopic observation of the culture dishes after 42 days of differentiation (blue circles: quantified areas) and (C) quantification of the pigmented area (n = 3, mean ± SD), (D) Representative immunofluorescence images for the RPE markers PAX6 and MITF after 42 days of differentiation. Nuclei are stained with DAPI (blue).