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. 2019 Jul 23;9:10646. doi: 10.1038/s41598-019-47123-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Automated differentiation and amplification of a pure population of hESC-RPE cells without manual selection. (A) Representative immunofluorescence and quantification for the RPE markers MITF and PAX6 at passage 2 after 21 days of culture. Nuclei are stained with DAPI. (B) Relative gene expression of RPE markers quantified by RT-qPCR (n = 3, mean ± SD). (C) Representative flow cytometry histogram for the pigmentation marker TYRP1.