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. 2019 Jul 23;9:10646. doi: 10.1038/s41598-019-47123-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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hESC-RPE cells obtained by automated differentiation can be maintained in culture until passage 3 before starting an EMT. (A) Light microscopy images of hESC-RPE cells at passage 3, 4 and 5 at day 21. (B) Relative gene expression of EMT (LUM and FN1) and RPE (MITF and BEST) markers quantified by RT-qPCR (n = 3, mean ± SD).