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. 2019 Jul 23;9:10689. doi: 10.1038/s41598-019-47069-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Silencing the NMT1 gene blocked the infection of human MDM by HIV-1 CRF02_AG isolates, but had no effect on clade-B HIV-1 infection. Silencing of NMT1, NMT2, CKIIα and CKIIβ genes in primary human MDM was confirmed by RT-PCR (A) and Western blot analyses (B). For panels A and B, controls are non-transduced MDM. “S” are MDM transduced with scrambled shRNA. Following NMT1 and NMT2 gene silencing, MDM were infected with CRF02_AG or clade-B HIV-1 isolates (3 different isolates for each subtype, each isolate tested in triplicate) and RT activity measured from day-5 to day-21 p.i. (C,D) RT activity in MDM with NMT1 gene silenced. (E,F) RT activity in MDM with NMT2 gene silenced. For panels C–F, controls are non-HIV infected (mock-infected) MDM, “S” are MDM transduced with scrambled shRNA; “sh” are MDM transduced with NMT1 or NMT2 shRNA. **P < 0.01; ^#P < 0.0001, compared to infected MDM treated with similar concentrations of scrambled shRNA (S).