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. 2019 Jul 23;9:10674. doi: 10.1038/s41598-019-47059-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) The representative normalized turbidimetric gelation kinetics of ECM, ECM/G and ECM/D crosslinked by 1 mM and 10 mM genipin or EDC. (B) Time to reach 50% and (C) 95% maximal absorbance. (D) Lag time of ECM hydrogels determined as an intercept point of the slope at log t_1/2 and turbidimetry baseline with 0% absorbance. (E) The gelation rate delta S defined as the slope of the linear region of the gelation curve (*p < 0.05, **p < 0.01, n = 3).