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. 2019 Jul 23;9:10645. doi: 10.1038/s41598-019-46378-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Structure of zebrafish ar gene and DNA construct for liver-specific knockout. (A) Schematic representation of eight ar exons to cover the complete coding region. The sequence (underlined) and position of the ar gRNA target at the first exon are indicated. PAM sequence (Cas9 binding site) is in Red. (B) Schematic illustration of the liver-specific CRISPR construct. Tol2, Tol2 transposon; P_cmlc2: cmlc2 promoter; ar gRNA, ar guide RNA; pA: poly A signal; P_fabp10: fabp10a promoter. (C) The negative (left) and positive (right) F1 larvae from an L-ARKO founder transgenic zebrafish. The heart is marked with GFP expression as a screening marker.