. 2019 Jul 23;10(4):e01146-19. doi: 10.1128/mBio.01146-19

TABLE 1.

Kinetic parameters of DPCK toward dephospho-CoA and GTP^a

Source	Substrate	V_max (μmol min⁻¹ mg⁻¹)	K_m (mM)	k_cat (s⁻¹)	k_cat/K_m (s⁻¹ mM⁻¹)
T. kodakarensis	Dephospho-CoA	17.0 ± 0.8	0.14 ± 0.02	5.57	40.4
	GTP	20.4 ± 1.0	0.26 ± 0.06	6.68	25.7
M. tuberculosis	Dephospho-CoA	—	0.035	0.029	0.83
	ATP	—	0.057	0.048	0.84
E. coli	Dephospho-CoA	—	0.14	—	—
	ATP	—	0.74	—	—
E. histolytica (EhDPCK1)	Dephospho-CoA	3.71	0.11	1.48	13.5
	ATP	3.54	0.020	1.41	70.5
E. histolytica (EhDPCK2)	Dephospho-CoA	2.48	0.058	0.96	16.5
	ATP	2.71	0.015	1.05	70

Reaction temperature for T. kodakarensis DPCK was 80°C. Values for the enzymes from M. tuberculosis, E. coli, and E. histolytica were obtained from previous studies (32, 36, 40). —, not reported.