TABLE 2.

Sequences of primers used in this study

Primer name	Primer sequence
TK1334F	5′-GGGCCATATGATAGTCATAGTCACTGGAATGC-3′
TK1334R	5′-AAAAGGATCCTCAAAGCTTCGAGATAATTTCAT-3′
TK1697F	5′-AAAGGATCCCATATGAAAATGTTCTTCAGGCTT-3′
TK1697R1	5′-AAAGAATTCTTAATCTCCATCACGAACCA-3′
TK1697R2	5′-AAAGTCGACTTAATCTCCATCACGAACCA-3′
TK2192F	5′-TGGCGCATATGGAGGCGGGGAAGATGATAATAGGCG-3′
TK2192R	5′-ATGAGAATTCTCATCCCATTACCTCCGAAATTATCTCCT-3′
DTK0517F	5′-CATCGGCGAGAACGACCGCT-3′
DTK0517R	5′-CGTGCTCCTGACTGTGTGAAAGAG-3′
DTK1697F	5′-CTGGAAGGACATAGATGACGTTCCAAAGGC-3′
DTK1697R	5′-ATCCGCCATGGACGCCCGACAGGATAAGCC-3′
DTK2128F	5′-GCTTGTCGGTTGCCAGAACAC-3′
DTK2128R	5′-CCTTCGGGCACTATGCGGTG-3′
DTK0517invF	5′-CCCGGTTTTATCTTTTATCGCTTATTC-3′
DTK0517invR	5′-CTGAATCACCGTAGGTAGTTAGAAAAAGGG-3′
DTK1697invF1	5′-ATGGAGATTAAGGTTACCGA-3′
DTK1697invR1	5′-TTTCACCTTACCCTGATGGC-3′
DTK1697invF2	5′-AAGAGAAAAGAATACTCCCCCGA-3′
DTK1697invR2	5′-TTCTCTAGTAAGCCTGAAGAACATTTT-3′
DTK2128invF	5′-GCCCAAAGGTGATAACACTA-3′
DTK2128invR	5′-GGTCCTACTTACTCATGCAGACCAATAA-3′
TK1697(D48A)F	5′-GCCGTCGTCACGGAGAACGTCCT-3′
TK1697(D48A)R	5′-CCCAACAGTGACAACGGGGTGTTT-3′
TK1697(D67A)F	5′-GCCCTCAAGACGAAGAGAAAAGAATAC-3′
TK1697(D67A)R	5′-GTAAAGGGCAATTATTGGTTTGACGC-3′
TK1697(D125A)F	5′-GCCTTGGCGGCGATCCCGG-3′
TK1697(D125A)R	5′-CTCCTCCCCGCTGACCAGGATGT-3′