FIG 5.
AnvM directly bound to TLR2/5 and regulated genes associated with flagella as well as LPS. (A and B) A bacterial two-hybrid experiment demonstrated an interaction between AnvM and TLR2 or TLR5. The recombinant strain harboring different proteins was separately streaked on nonselective and dually selective media. The strain expressing LGF2 and GAI11P was used as a positive control. (C) RT-qPCR was performed to measure the transcription level of lptD under aerobic conditions. (D) RT-qPCR revealed that AnvM spurred expression of flip, fliF, and dnpA. The experiments were repeated at least three times, and similar results were observed. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (comparing the ΔanvM strain with the wild-type strain using Student's t test). Data are representative of results from three independent experiments.