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. 2019 Jul 17;10:1660. doi: 10.3389/fimmu.2019.01660

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Copyright © 2019 Zhang, Chen, Zhao, Li, Ni, Chen and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Nfatc4 was expressed in the cochlear hair cells, and Nfatc4^−/− mice showed normal cochlear development and hearing function. (A) Nfatc4 immunofluorescence staining in the cochlear hair cells (middle turns) of P2 and P30 WT mice. Myo7a and Sox2 were used as hair cell and supporting cell markers, respectively. (B) Myo7a immunofluorescence in cochlear hair cells (middle turn) of adult Nfatc4^−/− and WT mice. (C) The hearing thresholds of ABR measurement in the adult Nfatc4^−/− and WT mice. (D) The numbers of hair cells in Nfatc4^−/− and WT mice. Scale bars: 25 μm. n = 5.