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. 2019 Jul 17;9:255. doi: 10.3389/fcimb.2019.00255

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Copyright © 2019 Ares, Sansabas, Rodríguez-Valverde, Siqueiros-Cendón, Rascón-Cruz, Rosales-Reyes, Jarillo-Quijada, Alcántar-Curiel, Cedillo, Torres, Girón and De la Cruz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cholesterol affects the production of capsular polysaccharide in K. pneumoniae. (A) Mucoviscosity of K. pneumoniae grown in LB with no supplement (LB), 0.05% tyloxapol (LBT), and 0.05% tyloxapol plus 50 μM cholesterol (LBC). The mucoviscosity was determined by low speed centrifugation and is expressed as OD_600nm of the supernatant. (B) Capsule quantification by determination of the glucuronic acid concentration from capsular polysaccharides. Statistically significant with respect to the WT bacteria grown in ** p < 0.01 between LB medium and ***p < 0.001.