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. 2019 Jul 17;9:255. doi: 10.3389/fcimb.2019.00255

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Copyright © 2019 Ares, Sansabas, Rodríguez-Valverde, Siqueiros-Cendón, Rascón-Cruz, Rosales-Reyes, Jarillo-Quijada, Alcántar-Curiel, Cedillo, Torres, Girón and De la Cruz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RcsA and H-NS are involved in the cholesterol-mediated capsule repression. Fold change expression (RT-qPCR) of galF (A), wzi (B), and manC (C) genes in the presence of cholesterol [LB with no supplement (LB), 0.05% tyloxapol (LBT), and 0.05% tyloxapol plus 50 μM cholesterol (LBC)] in different backgrounds: wild-type, ΔrcsA and Δhns. 16S rRNA was used as a reference gene for normalization. (D) Effect of cholesterol on phagocytic uptake of K. pneumoniae wild-type, ΔrcsA, and Δhns by THP-1 macrophages. Data represent the mean of at least three independent experiments performed in triplicates (mean ± SD). Statistically significant with respect to the WT bacteria grown in LB medium ***p < 0.001.