Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 17;10:1581. doi: 10.3389/fmicb.2019.01581

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Rodman, Martinez, Fung, Nakanouchi, Myers, Harris, Dang, Fernandez, Liu, Mendoza, Jimenez, Nikolaidis, Brennan, Bonomo, Sieira and Ramirez.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

PF induces changes in expression of genes involved in metabolism. (A) Genes differentially expressed by PF linked to the TCA cycle and GABA shunt. Red words and arrows depict statistically significant downregulated genes (adjusted P < 0.05). Green words and arrows depict statistically significant upregulated genes (adjusted P < 0.05). Dotted arrows indicate DEGs that were not statistically significant. Black arrows indicate genes that were not differentially expressed under PF treatment. Dashed lines indicate a second substrate utilized in the enzymatic conversion to next metabolite of the pathway. (B) 2-oxoglutarate dehydrogenase (ODH) activity of the cell soluble extracts of three model strains, indicated by relative fluorescent units (RFU). RFU values were recorded with an excitation of 340 and emission of 460 to qualify NADH formation, indicating activity of the dehydrogenase. Statistically significant results (^∗P < 0.05; multiple t-test; n = 3–6). (C) Minimal media (MM) growth curves of strains A118, A42, and AB5075 in 0.2% malate and 0.2% fumarate with or without PF. Mann–Whitney statistical analysis was performed to analyze all growth assays (P < 0.05; n = 3). (D) Malate dehydrogenase (MDH) activity of cell soluble extracts of the three model strains of Acinetobacter baumannii. Statistically significant results (^∗P < 0.05; multiple t-test; n = 3–6).