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. 2019 Jul 23;9:10699. doi: 10.1038/s41598-019-46932-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cluster analysis of fresh and preserved species mixing samples. Cell clustering identified seven cell clusters (a) and was mainly driven by murine (Mm) and human (Hs) origin and the preservation method. DMSO cryopreserved (DMSO) cells clustered together with the fresh cells whereas the cells preserved by CellCover reagent at 4 °C (CC4) and −20 °C (CC20) as well as methanol fixation (MeOH) formed separate clusters for murine and human cells respectively (b,d). The fraction of cross-species transcripts per cell (“cell impurity”) was variable across the different protocols and species (c). W01, storage for one week; W15, storage for 15 weeks.