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. 2019 Jul 23;9:10699. doi: 10.1038/s41598-019-46932-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Pseudo-bulk gene expression correlation of fresh and preserved cells. Scatter plots show pairwise correlation of pseudo-bulk gene expression profiles from fresh cells and DMSO cryopreserved (DMSO) cells (a), methanol fixed (MeOH) cells (b) and cells preserved by CellCover reagent (c) at 4 °C (CC4) and −20 °C (CC20). Cells were stored for one week (W01) and 15 weeks (W15). Pearson correlation coefficient (R) indicates the degree of correlation. Data are shown for human HEK293 cells derived from the species mixing experiment. Axes represent log₂ (UMI + 1) counts.