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. 2019 Jul 23;9:10699. doi: 10.1038/s41598-019-46932-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immune cell populations in fresh and cryopreserved samples. Cluster analysis identified 13 cell clusters in immune cells isolated from rat liver (a). Cell clusters represent macrophages/Kupffer cells, natural killer/natural killer T cells (NK(T) cells), T cells, B cells, neutrophils, plasmacytoid dendritic cells (pDCs), cycling cells (Cell cycle) and cells with increased expression of mitochondrial transcripts (Mito. high, b). The relative abundance per cell population is shown for fresh and DMSO cryopreserved (DMSO) samples per negative control (NC) and positive control (PC) animal, respectively (c).