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. 2019 Jul 23;9:10673. doi: 10.1038/s41598-019-46764-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of ductular cells from AAF/CCL4-induced cirrhosis rat model. (A,B) Flow cytometry analysis of freshly isolated ductular cells from normal (A) and AAF/CCL4 (B) livers. The y-axis indicates EpCAM expression (PE fluorescence) and the x-axis represents side scatter (SSC). The positive and negative fractions of each liver were collected as described in the Materials and Methods. Percentage of cells expressing EpCAM is shown. (C) Identity of EpCAM-expressing cells in the positive fraction was further confirmed by staining for SOX9 and CK19.