Figure 1.

Comparison of Prodigy manufactured CAR T cells for cell expansion, viability, transduction, VCN, and subsets. (A) A schematic of the insert sequence of AIC100 lentiviral vector. SS = signal sequence; TM = transmembrane; Cyt = cytosolic domain; hSSTR2 = human SSTR2. DNA ruler is shown above features. (B,C) Cell expansion and viability were quantified manually by hemocytometer. CD3 and CD4/CD8 subsets, and CAR positive cells were analyzed by flow cytometer. VCN was determined by ddPCR. (D) CAR expression was determined by dual labeling of cells by anti-Myc and anti-SSTR2 antibodies for CD4/CD8 sorted cells (day 0) and T cells harvested from Prodigy (day 9, 10, or 11).