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. 2019 Jul 23;9:10634. doi: 10.1038/s41598-019-46938-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparable killing of target cells expressing human versus murine ICAM-1 by AIC100. (A) The affinity of soluble F292A to 293T cells stably transduced to express human and mouse ICAM-1 was determined by competition assay using flow cytometer. (B) Wild-type 293T cells and 293T cells transduced with human versus murine ICAM-1 were tested for ICAM-1 expression. Open and filled histograms correspond to unlabeled and anti-ICAM-1 antibody stained cells, respectively. Antibody clones used were HA58 (anti-human ICAM-1) and 3E2 (anti-murine ICAM-1). (C) E to T assay of AIC100 cells against ICAM-1 negative 293T, human ICAM-1 transduced 293T (hCD54), and murine ICAM-1 transduced 293T (mCD54). The ratio of T cells to target cells was 2.5:1.