Table 2.
AIC100 toxicity on cell lines and primary cells of human and mouse tissues.
| Target cells | %cell death by NT* | %cell death by CAR T* | significance by t-test** |
|---|---|---|---|
| Cell lines | |||
| 293T | 3.3 ± 1.3 (10) | 2.2 ± 0.6 (8) | n.s. |
| 293T(hCD54) | 7.4 ± 0.9 (8) | 35.4 ± 4.0 (10) | <0.0001 |
| 8505C | 3.2 ± 1.2 (8) | 22.6 ± 3.8 (10) | 0.0005 |
| HeLa | 5.7 ± 1.1 (9) | 40.6 ± 6.9 (10) | 0.0002 |
| Human primary cells | |||
| Lung Microvascular Endothelial Cells | −1.2 ± 1.5 (6) | −0.7 ± 1.4 (8) | n.s. |
| Kidney Glomerular Endothelial Cells | 1.1 ± 0.7 (4) | 6.1 ± 1.5 (7) | n.s. |
| Lung Alveolar Epithelial Cells | 3.4 ± 1.4 (7) | 8.4 ± 1.9 (10) | n.s. |
| Cardiac Myocytes | 1.9 ± 3.0 (4) | 5.4 ± 3.0 (5) | n.s. |
| NK cells | −12.9 ± 2.9 (4) | −14.5 ± 1.7 (5) | n.s. |
| B cells | −10.4 ± 0.3 (4) | −5.9 ± 1.9 (5) | n.s. |
| Mouse (BALB/c) primary cells | |||
| Aortic Endothelial Cells | −1.0 ± 2.2 (4) | 0.9 ± 2.3 (5) | n.s. |
| Kidney Glomerular Endothelial Cells | −8.4 ± 1.9 (4) | −1.0 ± 2.2 (5) | n.s. |
| Lung Alveolar Epithelial Cells | −0.8 ± 0.9 (6) | 0.3 ± 0.8 (8) | n.s. |
| Artery Smooth Muscle Cells | −1.5 ± 1.4 (4) | 0.7 ± 1.7 (4) | n.s. |
| Liver Epithelial Cells | 0.9 ± 0.3 (4) | 1.8 ± 1.1 (6) | n.s. |
| Cardiac Myocytes | 1.3 ± 1.9 (4) | 2.0 ± 2.4 (4) | n.s. |
*Percent cell death is shown as mean ± sem (sample size). Negative values indicate that spontaneous release of 51Cr from target with T cells is lower than from target only condition. **Student t-test (unpaired, two-tailed) to examine significance (p < 0.05) of cell death by non-transduced (NT) T cells versus CAR T cells at E to T ratio of 25:1. n.s. = not significant. P values are shown for significance.