TABLE 2.
Primers and probes designed to quantify AAVrh.10hCLN2 and AAVrh.10-mCherry vector.
| Target | Label | DNA sequence of the oligonucleotide (5′-sequence-3′) | Final concentration in the reaction (nmol/L) | References |
| CMV enhancer | FP-CMV | GTCAATGGGTGGAGTATTTACGG | 900 | De et al.,2018 |
| RP-CMV | GCATTATGCCCAGTACATGACCT | 900 | ||
| P-CMV | FAM-CAAGTGTATCATATGCCAAGTACGCCCCC-BkFQ | 300 | ||
| Beta actin | FP-beta actin | CCGCAGCCATTGCCTTT | 900 | This study |
| RP-beta actin | CCGCACAGATTTGGGACAA | 900 | ||
| P-beta actin | FAM-ATGGTAATCGTGCGAGAGGGCGC-BkFQ | 300 | ||
| Beta globin | FP-beta globin | TCAGGTGCAGGCTGCCTAT | 900 | This study |
| RP-beta globin | TTTGTGAGCCAGGGCATTG | 900 | ||
| P-beta globin | FAM-AGAAGGTGGTGGCTGGTGTGG-BkFQ | 300 | ||
| PolyA | FP-polyA | GATTTTTCCTCCTCTCCTGACTACTC | 900 | This study |
| RP-polyA | GCTGCAGGTCGAGGGATCT | 900 | ||
| P-polyA | FAM-CAGTCATAGCTGTCCCTCTTCTCTT-BkFQ | 300 |
FP, forward primer; RP, reverse primer; P, probe; FAM, fluorescence reporter; BkFQ, black hole fluorescence quencher.