TABLE 3.

Comparison of vector genome titer determined by qPCR and ddPCR on samples purified by CIM chromatography.

Partially purified		qPCR			ddPCR
downstream sample (number and treatment)	Buffer composition of the sample	Average quantity (vg/mL)	CV%	Bias of DNase to DNase+proteinase K	Average quantity (vg/mL)	CV%	Bias of DNase to DNase+proteinase K
1 – DNase	1.5 mM potassium phosphate, pH 8	3.8E+11	12	−42%	8.9E+10	3	−19%
1 – DNase+Proteinase K		6.6E+11	17		1.1E+11	4
2 – DNase	1.5 mM potassium phosphate, pH 8	2.6E+11	6	−54%	5.5E+10	5	−35%
2 – DNase+Proteinase K		5.6E+11	11		8.5E+10	7
3 – DNase	1.5 mM potassium phosphate, pH 8	1.7E+11	20	−62%	4.8E+10	2	−27%
3 – DNase+Proteinase K		4.5E+11	6		6.6E+10	6∗∗
4 – DNase	50 mM acetate, 100 mM NaCl, 0.5% Sucrose, 0.2% Poloxamer, pH 3.7	3.5E+10	10	−38%	6.7E+09	2∗∗	−17%
4 – DNase+Proteinase K		5.6E+10	7		8.1E+09	6
5 – DNase	50 mM acetate, 100 mM NaCl, 0.5% Sucrose, 0.2% Poloxamer, pH 3.7	6.9E+10	10	−37%	1.5E+10	3	0%
5 – DNase+Proteinase K		1.1E+11	8		1.5E+10	3
6 – DNase	20 mM Tris, 2 mM MgCl2, pH 8.0	4.4E+10	15	na	3.4E+10	7	na
7 – DNase	1.3 M potassium phosphate pH 7.0	3.0E+08*	42	na	4.1E+08	8	na
8 – DNase	0.7 M potassium phosphate pH 7.0	4.9E+09	15	na	4.2E+09	5	na
9 – DNase	1.5 M potassium phosphate pH 7.0	6.3E+09	23	na	5.8E+09	8	na
10 – DNase	1.5 M potassium phosphate pH 7.0	3.7E+08*	30	na	2.9E+08	12	na
11 – DNase	0.8 M potassium phosphate pH 7.0	1.6E+09	9	na	1.4E+09	8	na
12 – DNase	20 mM Tris, 2 mM MgCl2, pH 8.0	4.9E+10	9	na	4.8E+10	5	na
13 –DNase	1.1 M potassium phosphate pH 7.0	1.5E+10	9	na	1.2E+10	8	na
14 – DNase	1.1 M potassium phosphate pH 7.0	1.1E+09*	27	na	1.1E+09	12	na

Average quantity of the vector genome (vg/mL) was determined from nine to six replicates of qPCR and ddPCR, respectively, unless stated otherwise. ^*First dilution used in experiment was inhibited (six replicates used for CV% calculation), ^∗∗results for only one dilution due to saturated droplets (two replicates used for CV% calculation), na, not applicable.