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. 2019 Jul 10;286(1906):20191080. doi: 10.1098/rspb.2019.1080

Figure 3.

Figure 3.

Molecular and chromosomal abnormalities in interspecies hybrids. (a) Gene expression in male reproductive organs of An. coluzzii MOPTI, An. merus, F1 hybrids of ♀An. merus × ♂An. coluzzii MOPTI (F1 M × C) and F1 hybrids of ♀An. coluzzii MOPTI × ♂An. merus (F1C × M) analysed by RT-PCR. AgS7—an endogenous control gene. (b) Confocal images of primary spermatocytes with FISH showing pairing and unpairing of X (red) and Y (green) chromosomes in An. coluzzii MOPTI males and F1 hybrid males from the ♀An. merus × ♂An. coluzzii MOPTI cross. (c) Percentage of the X and Y chromosome pairing in primary spermatocytes of six An. coluzzii MOPTI males and six F1 hybrid males from the ♀An. merus × ♂An. coluzzii MOPTI cross. Statistical significance was assessed with a two-sample pooled t-test (p < 0.001).