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. 2019 Jul 17;10:796. doi: 10.3389/fphar.2019.00796

Figure 5.

Figure 5

Effects of BSYS-, BS-, and HTHX-containing serum on brain-derived neurotrophic factor (BDNF) and TrkB protein expression in SH-SY5Y cells. SH-SY5Y cells (5 × 107/dish) were seeded in 100-mm dishes in normal medium (10% FBS) for 48 h and then shifted to low-serum medium (0.25% FBS) prior to exposure to medicine-free serum or serum containing medicines from BSYS for an additional 24 h. BDNF and TrkB protein expression was detected by WB, as described in the Materials and Methods. Data represent the means ± SE, n = 4. *P < 0.05 vs. NC; # P < 0.05, ## P < 0.01 vs. MO.