Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 17;10:1661. doi: 10.3389/fimmu.2019.01661

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Gao, Shang, Zhang, Zhang, Zhang, Zhang and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Lnc-C/EBPβ promotes differentiation of PMN-MDSC but impedes Mo-MDSC in vitro. (A) Flow cytometry of inflammatory cytokine and tumor mediated CD11b⁺Ly6G⁺Ly6C⁻ and CD11b⁺Ly6G⁻Ly6C⁺ MDSCs. 1D8, mouse ovarian carcinoma; 4T1, mouse breast cancer; B16, mouse melanoma. BMC, bone marrow cells; GM, GM-CSF. (B) Flow cytometry of Gr1⁺CD11b⁺, Gr1^hiCD11b⁺, and Gr1^lowCD11b⁺ MDSCs in lnc-C/EBPβ knockdown or exogenous lnc-C/EBPβ treated MDSCs. The percentages and absolute number of cells were compared (n = 3). (C) Flow cytometry of CD11b⁺Ly6G⁺Ly6C⁻ and CD11b⁺Ly6G⁻Ly6C⁺ MDSC in lnc-C/EBPβ knockdown or exogenous lnc-C/EBPβ treated MDSCs. The percentages and absolute number of cells were compared (n = 3). (D) Flow cytometry of lnc-C/EBPβ nockdown and extinct lnc-C/EBPβ treated MDSCs. After culturing for 4 days, lnc-C/EBPβ knockdown and extinct lnc-C/EBPβ treated MDSCs were analyzed by staining using anti-Gr-1, anti-CD11b, and F4/80 antibody. The percentages and absolute number of cells were compared (n = 3). (E) QRT-PCR of mouse lnc-C/EBPβ in MDSCs after transfecting with lnc-C/EBPβ lentivirus (upper) and fluorescence microscopic imaging of green fluorescent protein in lentivirus infected cells (lower). (F) QRT-PCR of mouse lnc-C/EBPβ in cells transfected with different concentrations of lentivirus and flow cytometry of CD11b⁺ly6G⁺Ly6C⁻, CD11b⁺ly6G⁻Ly6C⁺ MDSCs in lnc-C/EBPβ lentivirus treated MDSCs. MOI, multiplicity of infection; kdLNC(kd), lentivirus/lnc-C/EBPβ shRNA; oeLNC(oe), lentivirus/lnc-C/EBPβ; kdNC and oeNC, control lentiviruses. Data are a representative of at least three experiments. Absolute number = total cell number × percentage of MDSC subsets. Two-tailed, paired T-test was used in B-F. *p < 0.05; **p < 0.05; ^***p < 0.001. NS, no significant.