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. 2019 Jul 17;10:1661. doi: 10.3389/fimmu.2019.01661

Figure 6.

Figure 6

Human lnc-C/EBPβ has a similar function with mouse. (A) QRT-PCR of IL4i1 in lnc-C/EBPβ knockdown human MDSC-like cells. (B) Immunoblotting of IL4i1 in lnc-C/EBPβ knockdown human MDSC-like cells. (C) Flow cytometry of Lin−HLA-DR−CD14+CD11b+ MDSCs subsets in healthy individuals and patients with colon and rectal cancer. (D) QRT-PCR of human lnc-C/EBPβ in MDSC subsets isolated from the human colon, rectal cancer and healthy individuals. (E) QRT-PCR of human lnc-C/EBPβ in MDSC subsets isolated from the human colon and rectal cancer. (F) QRT-PCR of human IL4i1 in MDSC subsets isolated from the human colon and rectal cancer. (G) Immunostaining and RNA-FISH in the human colon cancer and para cancer tissues. Red, CD11b; Green, lnc-C/EBPβ; Blue, nuclei. One representative of 12 patients with colitic cancer. Scale bar = 200 μM. (H) Immunostaining and RNA-FISH in the peripheral blood Lin−HLA-DR−CD33+CD11b+ MDSCs in the patients with colon cancer. Red, CD11b; Green, lnc-C/EBPβ; Blue, nuclei. One representative of 12 patients with colitic cancer. Scale bar = 200 μM. siNC, siRNA control; siLNC, lnc-C/EBPβ siRNA; shNC, empty lentiviruses; shLNC, lnc-C/EBPβ shRNA/lentiviruses. Error bars in (A,D–F) represent standard deviations from 3 independent measurements. Two-tailed, paired T-test was used in (A,D–F). *p < 0.05; **p < 0.05; ***p < 0.001. NS, no significant.