Figure 3.

Loss of TRESK increases activation in response to inflammatory stimulation. (A) Representative traces of Ca²⁺ signals in TRESK [KO] TG neurons exposed to inflammatory soup (IS; 1 min) followed by a 5 min wash out and then 50 mM KCl to depolarize all neurons (30 s). (B) Proportion of IB4⁺ TG neurons from each genotype responding to IS with a Ca²⁺ peak >0.2 ΔF/F (TRESK [WT] n = 45, TRESK [KO] n = 97 cells). (C) Patch clamp recordings of TG neuron firing in response to acute (5 min) exposure to IS. (D) Quantification of action potential discharge during the 5 min of inflammatory challenge. Data represents mean ± SEM from 10 recordings per group. Recordings were pooled from TG obtained from three mice (per genotype), cultured independently on three separate days. *p < 0.05, student’s unpaired t-test.