Table 1.
Minor phospholipids identified in human dermal fibroblasts by liquid chromatography electrospray ionisation tandem mass spectrometry (LC-ESI(−) MS/MS) and mass spectrometry (MS)3.
| Experimental Value (m/z) |
Adduct | Regiochemical Assignment | Molecular Formula (M) |
|---|---|---|---|
| Plasmenyl-phosphatidylcholine (p-PC) | |||
| 700.5286 | [M − CH3 − H]− | (16:0/16:1) | C39H75NO7P |
| 702.5448 | [M − CH3 − H]− | (16:0/16:0) | C39H77NO7P |
| Plasmanyl-phosphatidylcholine (o-PC) | |||
| 700.5286 | [M − CH3 − H]− | (16:0/16:2) | C39H75NO7P |
| 702.5448 | [M − CH3 − H]− | (16:0/16:1); (15:0_17:1) | C39H77NO7P |
| 714.5443 | [M − CH3 − H]− | (16:1_19:1) | C40H77NO7P |
| 716.599 | [M − CH3 − H]− | (18:1/15:0); (16:1/17:0) | C40H79NO7P |
| 718.5756 | [M − CH3 − H]− | (17:0_16:0) | C40H81NO7P |
| 732.5912 | [M − CH3 − H]− | (16:0/18:0); (18:0/16:0); (17:0/17:0) | C41H83NO7P |
| 752.5599 | [M − CH3 − H]− | (16:0/20:4) | C43H79NO7P |
| 756.5912 | [M − CH3 − H]− | (16:0/20:2) | C43H83NO7P |
| 762.5654 | [M + HCOO]− | (16:0/16:1) | C41H81NO9P |
| 780.5912 | [M − CH3 − H]− | (18:0/20:4) | C45H83NO7P |
| 802.5967 | [M + HCOO]− | (17:1/18:1) | C44H85NO9P |
| 812.5811 | [M + HCOO]− | (16:0/20:4) | C45H83NO9P |
| Plasmenyl-phosphatidylethanolamine (p-PE) | |||
| 672.4973 | [M − H]− | (16:0/16:1) | C37H71NO7P |
| 698.5130 | [M − H]− | (16:0/18:2) | C39H73NO7P |
| 700.5286 | [M − H]− | (16:0/18:1) | C39H75NO7P |
| 720.4973 | [M − H]− | (16:0/20:5) | C41H71NO7P |
| 722.5130 | [M − H]− | (16:1/20:4) | C41H73NO7P |
| 728.5599 | [M − H]− | (18:0/18:1) | C41H79NO7P |
| 748.5286 | [M − H]− | (18:1/20:4) | C43H75NO7P |
| 750.5443 | [M − H]− | (18:0/20:4) | C43H77NO7P |
| 772.5286 | [M − H]− | (18:1/22:6) | C45H75NO7P |
| 774.5442 | [M − H]− | (18:1/22:5) | C45H77NO7P |
| 776.5599 | [M − H]− | (18:0/22:5) | C45H79NO7P |
| Plasmanyl-phosphatidylethanolamine (o-PE) | |||
| 702.5443 | [M − H]− | (16:0/18:1) | C39H77NO7P |
| 716.5588 | [M − H]− | (17:0/18:2) | C40H77NO7P |
| 728.5599 | [M − H]− | (16:0/20:2) | C41H79NO7P |
| 762.5443 | [M − H]− | (17:0/22:5) | C44H79NO7P |
| 764.5599 | [M − H]− | (17:0/22:5) (19:0/20:5) | C44H79NO7P |
| 776.5599 | [M − H]− | (18:0/22:6) | C45H79NO7P |
| Phosphatidylserine (PS) | |||
| 700.5304 | [M − H]− | (18:1/12:2); (18:2/12:1) | C36H63NO10P |
| 702.5433 | [M − H]− | (18:0/12:2); (14:1/16:1) | C36H65NO10P |
| 732.482 | [M − H]− | (16:0/16:1); (18:1_14:0) | C38H71NO10P |
| 734.498 | [M − H]− | (16:1/16:1) | C38H73NO10P |
| 756.4820 | [M − H]− | (18:2_16:1); (18:1_16:2) | C40H71NO10P |
| 758.4980 | [M − H]− | (16:0/18:2); (18:1/16:1) | C40H73NO10P |
| 760.5149 | [M − H]− | 16:0/18:1; 16:1_18:0 | C40H75NO10P |
| 774.5303 | [M − H]− | (18:0/17:1); (18:1_17:0); (16:0_19:1); (16:1_19:0) | C41H77NO10P |
| 782.4977 | [M − H]− | (16:0/20:4); (18:2_18:2) | C42H73NO10P |
| 784.5134 | [M − H]− | (16:0/20:4); (18:1_18:3) | C42H75NO10P |
| 786.5309 | [M − H]− | (18:0/18:2); (18:1/18:1); (16:0/20:2); (16:1_20:1) | C42H77NO10P |
| 788.4977 | [M − H]− | (18:0/18:1); (16:0_20:1) | C42H79NO10P |
| 790.5532 | [M − H]− | (18:0/18:0) | C42H81NO10P |
| 796.5134 | [M − H]− | (17:0/20:4) | C43H75NO10P |
| 800.5447 | [M − H]− | (19:1_18:1) | C43H75NO10P |
| 806.4977 | [M − H]− | (16:0/22:6); (18:1_20:5) | C44H73NO10P |
| 808.5134 | [M − H]− | (16:0/22:5); (18:1_20:4); (18:0_20:5) | C44H75NO10P |
| 810.5297 | [M − H]− | (18:1/20:3); (18:0_20:4) | C44H77NO10P |
| 812.5450 | [M − H]− | (18:0/20:3); (18:1_20:2) | C44H79NO10P |
| 816.5760 | [M − H]− | (18:0/20:1); (18:1_20:0) | C44H81NO10P |
| 826.5603 | [M − H]− | (18:0/21:3) | C45H81NO10P |
| 832.5130 | [M − H]− | (18:0/22:7); (18:1_22:6) | C46H75NO10P |
| 834.5290 | [M − H]− | (18:0/22:6); (20:4_20:2) | C46H77NO10P |
| 836.5450 | [M − H]− | (18:0/22:5); (18:1_22:4); (20:1/20:4) | C46H79NO10P |
| 838.5603 | [M − H]− | (18:0_22:4) | C46H81NO10P |
| 840.5760 | [M − H]− | (18:0/22:3) | C46H83NO10P |
| 844.6070 | [M − H]− | (18:0/22:1); (16:0_24:1); (18:1_22:0) | C46H87NO10P |
| 856.5134 | [M − H]− | (20:3_22:6) | C48H75NO10P |
| 858.5290 | [M − H]− | (20:3_22:5) | C48H77NO10P |
| 860.5450 | [M − H]− | (20:0_22:7); (20:1/22:6) | C48H79NO10P |
Plasmalogen lipids are divided into plasmanyl-phospholipids (o-PL) which have an ether bond in position sn-1 to an alkyl group, and plasmenyl-phospholipids (p-PL) with an ether bond in position sn-1 to an alkenyl group. Legend: “/”: Fatty acyl chains positional isomeric level known; “_”: Regiochemistry not univocally established.