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Proposed inactivation mechanism of BlaC. The nucleophilic Ser-70 is deprotonated by Lys-73, which then attacks the phosphorus atom of bis(benzoyl) phosphate, and benzoate leaves. Glu-166 then deprotonates a catalytic water molecule, which attacks the phosphorus to displace the second benzoate, leading to the phosphorylation of Ser-70 and resulting in an aged form of the enzyme.
