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. 2019 Jul 9;24(13):2516. doi: 10.3390/molecules24132516

Figure 3.

Figure 3

Expression and antimicrobial assay of recombinant peocin in E. coli BL21(DE3). (A) SDS-PAGE analysis of protein from the E. coli BL21(DE3)/pET-peocin host; (B) The recombinant peocin was identified by Western blotting using a 6x-His epitope tag antibody. Lane M: protein marker; 1: total protein without IPTG induction; 2: total protein with IPTG induction at 37 °C; 3 and 5: insoluble proteins with IPTG induction at 37 °C and 27 °C, respectively; 4 and 6: soluble proteins with IPTG induction at 37 °C and 27 °C, respectively; (C) Antimicrobial activity assay of recombinant peocin from the lytic supernatant of E. coli BL21(DE3)/pET-peocin. Control and sample indicate the 20 µL of lytic supernatant from E. coli BL21(DE3)/pET-peocin without and with IPTG induction, respectively. A 10 µg of kanamycin was used as positive control.