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. 2019 Jun 27;20(13):3138. doi: 10.3390/ijms20133138

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Targeting and response of the NeonOxIrr indicator to external H₂O₂ in different compartments of live HEK293T mammalian cells. Confocal images of HEK293T cells transiently expressing the NeonOxIrr indicator in the cytoplasm (a), lumen of mitochondria (b), intermembrane space of mitochondria (c), nucleus (d), peroxisomes (e), plasma membrane (f), Golgi apparatus (g), and endoplasmic reticulum (h) are shown before and 5 min after H₂O₂ addition until a 200 µM final concentration. Scale bars – 20 µm.