Table 1.
In vitro characteristics of the NeonOxIrr indicator compared to HyPer3 and mEGFP.
| Protein | NeonOxIrr | HyPer3 | mEGFP | ||
|---|---|---|---|---|---|
| Red | Ox | Red | Ox | ||
| Absorbance maximum (nm) | 508 | 498 | 490 | ||
| Extinction coefficient (M−1cm−1)1 | ND | 110,000 | ND | 65,000 | 64,000 |
| Emission maximum (nm) | 520 | 518 | 512 | ||
| Quantum yield2 | 0.57 | 0.17 | 0.60 | ||
| Relative brightness (%) | ND | 170 | ND | 29 | 100 |
| pKa3 | 5.9 | 5.9 | ≥7.9 | ≥7.6 | 5.9 |
| pH-range (pH changes impact to contrast)4 |
pH 5.5–8.5 (6–52%) |
pH 5.5–8.5 (62–596%) |
NA | ||
| Maturation t0.5 at 37 °C (min)5 | 4.2 | 25 | 14 | ||
| Photobleaching t0.5 (s)6 | 168 ± 30 | 354 ± 3 | 174 ± 8 | ||
| Fluorescence contrast on bacterial suspension (fold)7 | 2.8 ± 0.6 | 2.5 ± 0.5 | NA | ||