Table 1.

Effect of supercritical fluid-carbon dioxide (SF-CO₂) extraction conditions on extraction yield and lipid recovery from Nannochloropsis sp. biomass.

Experimental Code	Extraction Yield (mg/g)	Extracted Total Lipids (mg/g)	Cumulative Lipids Purity (%)	Extracted FAs (mg/g)	Extracted FAs (%)	SFAs (mg/g)	MUFAs (mg/g)	PUFAs (mg/g)
E_01	20.9	5.60	26.79	5.33	4.84	1.53	1.95	1.85
E_02	55.92	6.70	11.98	6.21	5.64	2.11	2.32	1.78
E_03	56.64	9.19	16.23	8.12	7.44	2.57	2.96	2.67
E_04	40.66	8.85	21.76	7.60	6.90	4.78	1.53	1.29
E_05	66.42	9.38	14.12	8.90	8.08	2.66	2.80	3.45
E_06	75.84	11.70	15.42	10.13	9.19	3.13	3.64	3.36
E_07	14.74	2.25	15.29	0.095	0.09	0.04	0.03	0.03
E_08	50.82	10.24	20.15	9.67	8.78	3.17	3.48	3.02
E_09	74.3	12.51	16.83	11.69	10.61	3.28	4.11	4.29
E_10	43.86	8.07	18.41	7.41	6.73	2.65	2.74	2.03
E_11	58.26	10.37	17.79	9.26	8.41	2.93	3.59	2.74
E_12	74.72	11.69	15.65	11.19	10.16	3.32	4.05	3.82
E_13	51.22	9.66	18.86	7.86	7.14	4.69	1.48	1.70
E_14	77.92	12.27	15.75	11.67	10.59	3.27	4.44	3.96
E_15	79.90	14.13	17.69	13.45	12.21	3.93	4.72	4.80
E_16	16.38	5.84	35.67	4.23	3.84	1.86	0.58	1.79
E_17	72.78	14.30	19.65	13.74	12.47	4.23	5.12	4.40
E_18	94.28	18.39	19.51	17.56	15.94	4.74	5.89	6.92

Note: Standard deviation was less than 5% in all operative conditions. Where: T: Temperature; P: Pressure; CO₂ FR: CO₂ flow rate.