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. 2019 Jul 6;24(13):2482. doi: 10.3390/molecules24132482

Figure 4.

Figure 4

The effect of NAB on expression level of HO-1 in LPS-stimulated RAW264.7 cells. (A) Effect of NAB on HO-1 expression level in LPS-stimulated RAW264.7 cells. Cells were pretreated with NAB or positive control drug (dexamethasone, DEX) for 1 h and then stimulated with LPS (100 ng/mL) for 6 h. The total protein of the cells was prepared, and the expression level of HO-1 protein was measured by Western blotting. (B) Effect of NAB on the mRNA expression level in LPS-stimulated RAW264.7 cells. Cells were pretreated with NAB or positive control drug (dexamethasone, DEX, or sulforaphane, SFN) for 1 h and then stimulated with LPS (100 ng/mL) for 6 h. The total mRNA was prepared, and the expression level was evaluated by qRT-PCR. The density ratio of the LPS group (model control) was set to 1. Results are expressed as the mean ± SEM of three independent experiments. ** P < 0.01 and *** P < 0.001 vs. LPS-stimulated cells.