Table 4.
Quantitative analysis of the actin cytoskeleton organization was performed using the FilaQuant software (confocal images in Figure 3); *(mean ± s.e.m.; ANOVA post hoc Bonferroni, n = 10 cells; n.s. between 1 day vs 7 days laser process).
| Sample | Actin Filament Number | Total Filament Length (µm) | Mean Filament Length (µm) | Max. Filament Length (µm) | Orientation Dispersion (°) |
|---|---|---|---|---|---|
| Storage time 1 day | |||||
| Micro1 | 10.9 ± 2.9 a,b,c | 92.5 ± 38.5 a | 4.5 ± 0.7 a | 23.4 ± 9.4 | 17.5 ± 1.7 |
| Micro2 | 20.2 ± 4.3 | 105.4 ± 21.3 a | 5.6 ± 1.1 a | 18.1 ± 6.6 | 22.8 ± 2.2 |
| FLIPSS1 | 56.2 ± 14,6 | 387.5 ± 120.3 | 8.1 ± 1.1 | 33.5 ± 7.1 | 22.5 ± 2.0 |
| FLIPSS2 | 49 ± 8.9 | 359.2 ± 43.9 | 7.8 ± 1.4 | 39.4 ± 8.3 | 21.8 ± 1.3 |
| Reference | 49.5 ± 8.6 | 552.1 ± 89.5 | 10.4 ± 0.2 | 52.5 ± 10.7 | 21.5 ± 1.7 |
| Storage time 7 days | |||||
| Micro1 | 18.1 ± 3.2 b | 105.5 ±24.7 a,c | 3.7 ± 1.4 | 17.0 ± 5.6 | 24.5 ± 1.0 |
| Micro2 | 21.7 ± 2.6 | 104.4 ±11.9 a,c | 3.6 ± 1.2 | 15.5 ± 4.0 | 22.0 ± 1.7 |
| FLIPSS1 | 40.4 ± 4.1 | 213.7 ± 25.8 | 7.4 ± 4.3 | 40.3 ± 7.1 | 21.7 ± 2.1 |
| FLIPSS2 | 35.8 ± 5.1 | 427.5 ± 43.9 | 6.4 ± 3.7 | 46.5 ± 6.1 | 20.4 ± 1.3 |
| Reference | 35.7 ± 7.4 | 552.1 ± 89.5 | 6.7 ± 3.9 | 47.4 ± 13.3 | 22.4 ± 2.2 |
a: * p < 0.05 vs. Reference; b: * p < 0.05 vs. FLIPSS1; c: * p < 0.05 vs. FLIPSS2.