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. 2019 Jul 24;39(30):6012–6030. doi: 10.1523/JNEUROSCI.2950-18.2019

Figure 1.

Figure 1.

Development of a refined mechanical nociception assay using von Frey-like filaments in Drosophila larvae. A, Schematic of mechanical probe design and assay. B, Dose–response of aversive rolling versus increasing mechanical pressure. C, Latencies of aversive rolling upon application of the indicated mechanical pressures. D, Aversive rolling to mechanical probes as a function of the anatomical location stimulated. Diagram represents the approximate locations of thoracic segment 3 (T3) and abdominal segments 2 (A2) and 8 (A8). Both percentage response (left graph) and response latencies (right graph) are shown. E, Linear regression model relating mechanical pressure to nociceptive behavior. F, Nociceptive behavioral responses to mechanical probes exerting approximately equivalent force but differing pressures. G, Linear regression model relating applied pressure to nociceptive responses. H, Linear regression model relating applied force to nociceptive responses. Error bars indicate mean ± SEM. Two-tailed unpaired t test (F) and one-way ANOVA with post hoc Tukey's test (D) were used for statistical analysis: *p < 0.05; ***p < 0.001, ns, not significant.