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. 2019 Jul 4;20(13):3288. doi: 10.3390/ijms20133288

Figure 4.

Figure 4

Knockdown of dLipin leads to a reduced number of mitotic cells in wing pouch. The wing imaginal discs from 3rd-instar larvae of control (Sd > yw) and dLipin-kd flies (Sd > dLipin-IR265-272, Sd > dLipin-IR277-380) were stained with DAPI to visualize the DNA (b,e,h) and anti-PH3S10 antibody followed by anti-rabbit IgG Alexa FluorTM 488 antibody (a,d,g). Merged images of DAPI and PH3S10 antibody staining are shown (c,f,i). PH3S10-positive cells (mitotic cells) in the wing pouch of 3rd-instar larvae were counted using MetaMorph software (n = 10 for each genotype) (j). Dotted line indicates the wing pouch of the wing imaginal disc. Data are expressed as the means ± S.D. The statistical significance of the difference between control and dLipin-kd flies was evaluated using one-way ANOVA. ***, p < 0.01; Scale bar, 100 μm; IR, inverted repeat. Genotypes: Sd-GAL4/+; +; + (a–c), Sd-GAL4/+; UAS-dLipin-IR277-380/+; + (d–f), Sd-GAL4/+; UAS-dLipin-IR265-272/+; + (g–i).