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. 2019 Jul 4;20(13):3288. doi: 10.3390/ijms20133288

Figure 8.

Figure 8

Knockdown of dLipin activates the pro-apoptotic gene reaper. Wing imaginal discs from the 3rd-instar larvae of control and dLipin-kd flies that carry rpr-lacZ were stained with an anti-lacZ antibody (a,d,g) and with DAPI (b,e,h). Both images were merged (c,f,i). The fluorescence intensities in the wing pouch stained with anti-lacZ were analyzed using MetaMorph software (n = 14 for each genotype) (j). Data are expressed as the means ± S.D. The statistical significance of the difference between control and dLipin-kd flies was evaluated using one-way ANOVA. ***, p < 0.01; Scale bar, 100 μm; IR, inverted repeat. Genotypes: Sd-GAL4/+; +; rpr-lacZ/+ (a–c), Sd-GAL4/+; UAS-dLipin-IR277-380/+; rpr-lacZ/+ (d–f), Sd-GAL4/+; UAS-dLipin-IR254-476/+; rpr-lacZ/+ (g–i).