Figure 5.

The suppressive capacity of 1,25(OH)₂D₃-treated memory CCR6+ Th cells is retained in the presence of RA synovial fluid. Memory CCR6+ Th cells were sorted from healthy controls and cultured for 3 days with or without 1,25(OH)₂D₃. They were then used in the same suppression assay as for Figure 3, but in the presence of 20% synovial fluid. Proliferation for CD3+ T cells of six healthy donors (each depicted by its own symbol) is shown with or without suppressor cells, in the presence of three different synovial fluid samples (upper panel). Overall proliferation in synovial fluid is calculated from the average proliferation of T cells from each healthy donor in three different synovial fluids (lower panel). No suppr; no suppressors. Ctr-CCR6+; control-treated memory CCR6+ Th cells. 1,25-CCR6+; 1,25(OH)₂D₃-treated memory CCR6+ Th cells. Mean and SEM are shown for 6 healthy controls from 2 independent experiments. ^*p < 0.05.