Figure 5.
Effect of NE on sASC chondrogenesis. (A) Macroscopic, and histologic (sGAG) analysis as well as immunohistochemical type II collagen staining of untreated or NE-treated (10−9–10−6 M) chondrogenic sASC pellets at day 21 of differentiation (bars 500 μm). (B) Quantification of pellet volume and biochemical quantification of type II collagen and sGAG content of chondrogenic sASC pellets in the absence or presence of NE (10−9–10−6 M) at day 21. Each circle shows the mean of 3 replicates per patient (n = 6–10). Values are the percent of control (control = 100%, dashed line). Box plots are explained in legend to Figure 1. Significant p-values against untreated control are presented as * p < 0.05, ** p < 0.01, *** p < 0.001.