Figure 3.

TFP induced G0/G1 arrest in T24/R cells. (A) T24/R cells were separately treated with TFP (25 μM) and DMSO for 24 h. Cell cycle analyses were performed through flow cytometry with propidium iodide staining. Quantitative data are presented as means ± SD of three independents experiments, * p < 0.05 as compared with control. (B) T24/R cells were treated with TFP (12.5 or 25 μM) and DMSO for 24 h. The total cell lysates were assessed for the cyclin-dependent kinase inhibitors (CKIs): p21 and p27 by using Western blot analysis. Results shown are representative of at least three independent experiments.