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. 2019 Jul 1;20(13):3238. doi: 10.3390/ijms20133238

Figure 3.

Figure 3

Apoptosis change of annexin V/7AAD after EANT treatment. (A,B) Concentration effect of EANT on Annexin V/7AAD patterns and statistics. Breast cancer cells (MCF7 and SKBR3) were treated with control with DMSO only and EANT (15 and 25 μg/mL) for 24 h. Annexin V (+)/7AAD (−) and annexin V (+)/7AAD (+) were respectively regarded as early and later apoptosis. (C–F) Time course effect of EANT on Annexin V/7AAD patterns and statistics. Without or with (C,D) NAC pretreatment or (E,F) Z-VAD pretreatment, breast cancer cells (MCF7 and SKBR3) were treated with 25 μg/mL of EANT for 0 (control with DMSO only), 12, and 24 h. For each cell line, treatments labeled without the same lower-case letters indicate significant difference. p < 0.01~0.0001. Data, mean ± SD (n = 3). Positive controls for apoptosis are provided in Supplementary Figure S2C,D.