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. 2019 Jul 1;20(13):3238. doi: 10.3390/ijms20133238

Figure 5.

Figure 5

MitoSOX and MMP change after EANT treatment. (A,B) The concentration effect of EANT on MitoSOX patterns and statistics. Breast cancer cells (MCF7 and SKBR3 were treated with 0 (control with DMSO only), 15, and 25 μg/mL of EANT for 24 h. (C–F) Time course effect of EANT on MitoSOX patterns and statistics. Without or with (C and D) NAC pretreatment or (E,F) MitoTEMPO pretreatment, breast cancer cells (MCF7 and SKBR3) were treated with 25 μg/mL of EANT for 0 (control with DMSO only), 12, and 24 h. Annexin V (+) was counted to apoptosis. (G,H) The concentration effect of EANT on MMP patterns and statistics. Breast cancer cells (MCF7 and SKBR3) were treated with EANT for 24 h. (I,J) Time course effect of EANT on MMP patterns and statistics. Without or with NAC pretreatment, breast cancer cells (MCF7 and SKBR3) were treated with 25 μg/mL of EANT for 0 (control with DMSO only), 12, and 24 h. For each cell line, treatments labeled without the same lower-case letters indicate significant difference. p < 0.05~0.0001. Data, mean ± SD (n = 3). (+) or (−) respectively indicates the MitoSOX (+) or MMP (−) population. Positive controls for MitoSOX and MMP were provided in Supplementary Figure S2I–L.