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. 2019 Jul 9;20(13):3359. doi: 10.3390/ijms20133359

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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TSPO gene knockout using CRISPR/Cas9 system decreases respiration in human microglia cells. (A) Knockdown of TSPO did not alter respiration in C20 microglia cells (B) Oxygen consumption rate (OCR) was significantly reduced in several stages of respiration in TSPO KO cells (p < 0.001). (C) Basal respiration, maximal respiration, and ATP-related oxygen consumption were significantly decreased in TSPO knockout cells (p < 0.001). (D) Overexpression of TSPO in knockout cells resulted in increased respiration which did not differ from wt cells. (E) Basal respiration, maximal respiration, and ATP-related oxygen consumption which were reduced in KO cells, reached similar levels in TSPO overexpressing knockout cells.