Figure 1.

Effects of WM peptide on β amyloid (Aβ) deposition in 5×FAD mice. For 3 months, 2.5-month-old transgenic 5×FAD and wild-type female mice were fed a diet either containing or not containing 0.05% w/w WM peptide. Percentage of Aβ_1–42 positive area was detected by immunohistochemistry in the brains of the transgenic control mice [WM(−)] and transgenic mice fed on a diet containing WM peptide [WM(+)]. (A), (B), (D) and (E): The representative immunohistochemistry images including olfactory-entorhinal cortex and hippocampus for Aβ_1–42 in transgenic mice with or without WM peptide, respectively. (C) and (F): Semiquantification for Aβ_1–42 detected immunohistochemically in cortex and hippocampus, respectively. (G) and (H): The levels of Tris-buffered saline (TBS)-soluble Aβ_1–42 and TBS-insoluble and TBS-T-soluble Aβ_1–42, respectively, in the frontal cortex and hippocampus were measured by ELISA. Scale bars indicate 500 μm (A and B)and 400 μm (D and E), respectively. Data represent the mean ± SEM values. There were 5–7 mice in each group for immunohistochemistry and 9 control transgenic mice and 11 transgenic mice were fed on a diet containing the WM peptide for ELISA. The p-values shown in the graph were calculated via the Student’s t-test. * p < 0.05 and ** p < 0.01.