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. 2019 Jul 5;20(13):3301. doi: 10.3390/ijms20133301

Figure 4.

Figure 4

ELISA (enzyme linked immunosorbent assay) of (A) sICAM-1 and (B) sE-selectin (pg/mL) after stimulation of the inverted coculure CCinc (ISO-HAS-1/Caco-2) with aSNPs and 3C (cytokine mixture (IL-1β (100 U/mL), TNF-α (600 U/mL) and IFN-γ (200 U/mL)) for 48 h; uc: untreated control (without aSNP and 3C); aSNPs: -plain, 30 nm: Sicastar Red with a diameter of 30 nm, without surface modifications; -plain, -NH2, -COOH, 70 nm: Sicastar Red with a diameter of 70 nm and different surface modifications. All aSNPs were applied at a concentration of 100 µg/mL. Data are depicted as means ± SE of three independent experiments with n = 3. For statistical analysis one-way ANOVA with Dunnett’s Multiple Comparison test was conducted. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001; #: comparison within the group without 3C (dark grey columns, no significance observed); *: comparison within the group with 3C (light grey columns).