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. 2019 Jun 26;20(13):3113. doi: 10.3390/ijms20133113

Figure 1.

Figure 1

Effect of calcium concentration on the localization of desmogleins in human telomerase reverse transcriptase (hTert) cells. The cells were grown on coverslips in Keratinocyte growth medium (KGM) with 0.05 mM calcium for at least four days, and then shifted to 2 mM calcium for 24 h. After methanol (MeOH) fixation, the cells were stained with anti-desmoglein (Dsg) antibodies and fluorochrome coupled secondary antibodies (anti-mouse Alexa488, green). The coverslips were mounted in a mounting medium with 4′,6-diamidino-2-phenylindole (DAPI, blue) to visualize nuclei. n = 4 independent experiments, scale bar 20 µm.